Birthdate

03.10.1946

Organization

Republican Research and Practical Center for Epidemiology and Microbiology of the Health Ministry of the Republic of Belarus

Position

Head of the Laboratory of Clinical & Experimental Microbiology at the Republican Research & Practical Center for Epidemiology and Microbiology (RRPCEM) of the Ministry of Health, Republic of Belarus

Research specialization

Research area

Immunology

Research specialization

I. Basic Immunology. Natural Immunity. Mechanisms of natural resistance and antigen non-specific immunoreactivity, as the most essential and unique form of organism protection. The regularities of complement system functioning, the role of its components in numerous infections, allergic and autoimmune diseases, functional and biochemical methods of complement system studies. Autoantibodies, natural antibodies, Th1/Th2 regulation.

II. Ecologic Immunology. Influence of ecologic factors of biologic, physical and chemical nature on human immune system. Damages in the immune system of healthy individuals as a decisive point to form immunodependent pathology. Effects of small radiation doses on immune status in early and late post-Chernobyl accident periods. Immune system functions in children, residing on radionuclide-contaminated territories, methods of immunocorrection.

III. Clinical and Experimental Immunology. Functions of T-system immunity, immunoregulation; mechanisms of hypersensitivity (delayed type) formation as an effector link in the cellular immunity. Individual immunoreactivity (antigen specific response/non-responsiveness) of the organism to bacterial antigens and mitogens, which allow to increase diagnosis efficacy in infectious diseases (AIDS, hepatitis B & C, tuberculosis, klebsiella, chlamydiosis, trichinellosis etc.); methods of immune reactivity estimation and new approaches to immunocorrection, tumor immunology - immunology of lung cancer.

IV. Bacteriology. Clinical bacteriology (anaerobic bacteria – Clostridium difficile, bacteroides, gram-negative bacteria – family of Enterobacteriacceae) and Corynebacterium diphtheriae. Hospital infections monitoring of the resistance bacteria to antibiotics). Molecular-genetic investigation of the Mycobacteria tuberculosis infection.

V. Virology. Viral hepatitis B and C, rabies, HIV, measles, human enteroviruses, polioviruses, arboviruses (West Nile virus).

Career

09.2009-up till now -- Head of the Laboratory of Clinical & Experimental Microbiology at the Republican Research & Practical Center for Epidemiology and Microbiology (RRPCEM) of the Ministry of Health, Republic of Belarus
03.1995-09.2009 -- Director of the Research Institute for Epidemiology and Microbiology (RIEM) of the Ministry of Health, Republic of Belarus.
1999-2006 -- Head of the Division of Microbiology & Immunology at the Research Institute for Epidemiology and Microbiology (RIEM) of the Ministry of Health, Republic of Belarus.
1988-2006 -- Head of the Department of Microbiology, Immunology, Virology at Belarusian State Medical University in Minsk.
1987-1988 -- Head of the Laboratory of Children Immunology at State Medical Institute in Minsk.
1986-1987 -- Docent of the Department of Microbiology, Immunology, Virology at State Medical Institute in Minsk.
1979-1986 -- Assistant of the Department of Microbiology, Immunology and Virology at State Medical Institute in Minsk.

Scientific activities

Projects

1986 -- Head of the Programme of the investigations of radiation effects on children"s immune system and immunological monitoring of children, residing on radionuclide-contaminated territories.
1990-1992 -- National Immunization Programme and Programme of Immunologic Services Development were designed.
1994 -- International Medical Commission in the city of Bhopal (India) to evaluate effects of a gas disaster (1984) on the health of victims (immunoecological part of the project - tuberculosis and immune system function).
1996-2000 -- Head of the State Scientific-Technical Programme "Infectious Diseases".
1998-2000 -- Co-partner from Belarus side in the EC international project ”Optimization of prevention and surveillance of poliomyelitis and other enterovirus infections” (IC15-CT96-0912).
1999-2001 -- Co-partner from Belarus side in the EC international project ”Microbiological surveillance of diphtheria in the Eastern Europe” (IC15-CT98-0302).
2000-2003 -- Co-partner from Belarus side in the EC international project ENVRAD “Long-term effects of DNA damage in persons exposed to radioactively contaminated environment” (ICA2-CT-2000-10047)
2001-2010 -- Head of the State Scientific-Technical Programme “Infectious Diseases and Medical Biotechnology”
2001-2004 -- Co-partner from Belarus side in the Swiss National Science Foundation international project (SCOPES 2001-2004) “Autoantibodies, a link between chronic infectious and autoimmune diseases. AntiC1q autoantibodies: friend or foe?” (7BYPJ065654.1)
2002-2004 -- Co-partner from Belarus side in the EC international project “Study of the role of non-toxigenic Corynebacterium diphtheriae in the epidemiology of diphtheria” (INTAS-2001-2289)
2006-2009 -- Co-partner from Belarus side in the EC international project “The role of human genetic factors in the immune control of HIV-1: analysis of unique cohort of a single-source HIV-1 outbreak eliminates confounding effects of virus genetic diversity” (INTAS Ref. Nr 05-1000004-7749).

Publications

1. Khrustalev, V.V., Arjomandzadegan, M., Barkovsky, E.V., Titov, L.P. (2012) Low rates of synonymous mutations in sequences of Mycobacterium tuberculosis GyrA and KatG genes. Tuberculosis (Edinb). Apr 20. [Epub ahead of print] PubMed PMID: 22521568.
2. Arjomandzadegan, M., Sadrnia, M., Surkova, L.K., Titov, L.P. (2011) Study of promoter and structural gene sequence of whiB7 in MDR and XDR forms of Mycobacterium tuberculosis. West Indian Med. J., 60(3):251-6.
3. Bostanabad, S.Z., Nojoumi, S.A., Jabbarzadeh, E., Shekarabei, M., Hoseinaei, H., Rahimi, M.K., Ghalami, M., Pourazar Dizji, S., Sagalchyk, E.R., Titov, L.P. (2011) High level isoniazid resistance correlates with multiple mutation in the katG encoding catalase proxidase of pulmonary tuberculosis isolates from the frontier localities of Iran. Tuberk Toraks., 59(1):27-35.
4. Arjomandzadegan, M., Owlia, P., Ranjbar, R., Farazi, A.A., Sofian, M., Sadrnia, M., Surkova, L.K., Titov, L.P. (2011) Rapid and simple approach for identification of Mycobacterium tuberculosis and M. bovis by detection of regulatory gene whiB7. Acta Microbiol. Immunol. Hung., 58(1):65-74.
5. Arjomandzadegan M, Owlia P, Ranjbar R, Farazi AA, Sofian M, Sadrnia M, Ghaznavi-Rad E, Surkova LK, Titov LP. Prevalence of mutations at codon 463 of katG gene in MDR and XDR clinical isolates of Mycobacterium tuberculosis in Belarus and application of the method in rapid diagnosis. Acta Microbiol Immunol Hung. 2011 Mar;58(1):51-63.
6. Cherniakova, Iu.M., Pinchuk, L.S., Titov, L.P. (2011) [Impact of a magnetic field on blood separation kinetics in patients with joint diseases]. Klin Lab Diagn. Jan;(1):25-32. [In Russian].
7. Setareh, M., Titov, L.P., Surkova, L.K. (2009) High level association of mutation in KatG315 with MDR and XDR clinical isolates of Mycobacterium tuberculosis in Belarus. Acta Microbiol. Immunol. Hung., 56(4):313-325.
8. Flekhter, O.B., Medvedeva, N.I., Tolstikov, G.A., Galin, F.Z., Iunusov, M.S., Huong, N.T., Le, V.T., Svinova, O.V., Boreko, E.I., Titov, L.P., Glukhov, I.V. (2009) [Synthesis of olean-18(19)-ene derivatives from betulin]. Bioorg. Khim., 35(2):253-259. [In Russian].
9. Poleshchuk, N.N., Rubanik, L.V., Kapitulets, N.N., Kvacheva, Z.B., Kostiuk, S.A., Chernosheĭ, D.A., Kapitulets, S.P., Titov, L.P. (2008) [Biological and immunogenic characteristics of Chlamydia trachomatis strain MT-2A (serovar D) and its use for development of experimental inactivated vaccine]. Zh. Mikrobiol. Epidemiol. Immunobiol., (3):39-44. [In Russian].
10. Bostanabad, S.Z., Titov, L.P., Bahrmand, A., Nojoumi, S.A. (2008) Detection of mutation in isoniazid-resistant Mycobacterium tuberculosis isolates from tuberculosis patients in Belarus. Indian J. Med. Microbiol., 26(2):143-147.
11. Zakerbostanabad, S., Titov, L.P., Bahrmand, A.R. (2008) Frequency and molecular characterization of isoniazid resistance in katG region of MDR isolates from tuberculosis patients in southern endemic border of Iran. Infect. Genet. Evol., 8(1):15-19.
12. Zaker Bostanabad, S., Titov, L.P., Slizen, V.V., Taghikhani, M., Bahrmand. A. (2007) katG mutations in isoniazid-resistant strains of Mycobacterium tuberculosis isolates from Belarusian patients. Tuberk. Toraks., 55(3):231-237.
13. Kolodkina, V.L., Titov, L.P., Sharapa, T.N., Drozhzhina, O.N. (2007) [Point mutations in tox promoter/operator and diphtheria toxin repressor (DTXR) gene associated with the level of toxin production by Corynebacterium diphtheriae strains isolated in Belarus]. Mol. Gen. Mikrobiol. Virusol., (1):22-29. [In Russian].

Patents

2005 “Predicting method of septic complications in surgical patients”, Belarus national patent # 7482
2007 “Differential medium for isolation of intestinal yersiniosis and pseudotuberculosis”, Belarus national patent # 9828
2007 “Nutrient medium for detection of intestinal yersiniosis and pseudotuberculosis”, Belarus national patent # 9829
2007 “Means to suppress the herpes virus in cell culture”, Belarus national patent # 9985
2009 “Differential-diagnostic medium for the isolation of pathogenic and opportunistic bacteria”, Belarus national patent # 11975
2009 “Method of diagnosing the state of T-cell immunity in patients with viral hepatitis B, C and mixed hepatitis B + C”, Belarus national patent # 11965
2009 “Inductor for maturation of monocyte-derived dendritic cells in vitro”, Belarus national patent # 12361
2009 “The combination of the two CpG oligonucleotides from the genome of bacteria of the genus Klebsiella, causing the in vitro maturation of monocyte-derived dendritic cells”, Belarus national patent # 12704
2011 “Way to improve antichlamydial antibody genesis”, Belarus national patent # 14461
2011 “The medium for the cultivation of Campylobacter”, Belarus national patent # 14469
2011 “Activated by infrared light means for suppression of staphylococcal and fungal activity”, Belarus national patent # 15152
2012 “Test system for determination of diphtheria toxin”, Belarus national patent # 15675
2012 “Preparation method of autologous monocyte-derived dendritic cells for tuberculosis immunotherapy”, Belarus national patent # 15969

Contact data

Republican Research & Practical Center for Epidemiology & Microbiology (RRPCEM)
23 Filimonova St., 220114, Minsk, Republic of Belarus
Telephone: +375-172 37 69 98
Fax: +375-172 67 30 93
E-mail: leonidtitov@tut.by
http://www.belriem.by